Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, T-47D cells, HL-60 cells, Jurkat cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85707-1-RR targets KIF14 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | KIF14 fusion protein Ag23219 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 14 |
| Observed molecular weight | 186 kDa |
| GenBank accession number | BC113742 |
| Gene Symbol | KIF14 |
| Gene ID (NCBI) | 9928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15058 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF14 (Kinesin Family Member 14) is a microtubule-based motor protein belonging to the kinesin-3 family. It plays essential roles during cell division and has been implicated in various biological processes, including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. KIF14 is highly conserved across species and is characterized by a catalytic motor domain that binds to microtubules and ATP, enabling it to convert chemical energy into mechanical work. KIF14 interacts with the Skp1/Cul1/F-box (SCF) complex, particularly Skp2 and Cks1, to regulate the ubiquitination and degradation of p27Kip1. Knockdown of KIF14 results in the accumulation of p27Kip1, leading to cell cycle arrest and apoptosis.(PMID: 34131133, PMID: 24854087)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF14 antibody 85707-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |