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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF13A Polyclonal antibody

KIF13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27210-1-AP

Synonyms

KIF13A, kinesin family member 13A, Kinesin like protein KIF13A, Kinesin like protein RBKIN, RBKIN

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 27210-1-AP

    WB analysis of HepG2 using 27210-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27210-1-AP (KIF13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27210-1-AP

    IHC staining of human liver cancer using 27210-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27210-1-AP (KIF13A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27210-1-AP is KIF13A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF13A fusion protein Ag24356 相同性解析による交差性が予測される生物種
Full Name kinesin family member 13A
Calculated molecular weight 1805 aa, 202 kDa
Observed molecular weight 202 kDa
GenBank accession numberBC127115
Gene symbol KIF13A
Gene ID (NCBI) 63971
RRIDAB_2880802
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIF13A antibody 27210-1-APDownload protocol
IHC protocol for KIF13A antibody 27210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols