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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF12 Polyclonal antibody (12035-1-AP)

    WB analysis using 12035-1-AP (same clone as 12035-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using KIF12 Polyclonal antibody (12035-1-AP)

    WB analysis of Y79 using 12035-1-AP (same clone as 12035-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human kidney using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human kidney using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human renal cell carcinoma using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human renal cell carcinoma using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human pancreas cancer using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12035-1-AP (KIF12 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human pancreas cancer using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KIF12 Polyclonal antibody (12035-1-AP)

    IHC staining of human pancreas cancer using 12035-1-AP (same clone as 12035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12035-1-PBS in a different storage buffer formulation.

KIF12 Polyclonal antibody, PBS Only

KIF12 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12035-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Kinesin like protein KIF12, Kinesin-like protein KIF12, RP11 56P10.3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12035-1-PBS targets KIF12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2707

Product name: Recombinant human KIF12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-334 aa of BC010626

Sequence: MQRTFAWLLDRVQHLGAPVTLRASYLEIYNEQVRDLLSLGSPRPLPVRWNKTRGFYVEQLRVVEFGSLEALMELLQTGLSRRRNSAHTLNQASSRSHALLTLYISRQTAQQMPSVDPGEPPVGGKLCFVDLAGSEKVAATGSRGELMLEANSINRSLLALGHCISLLLDPQRKQSHIPFRDSKLTKLLADSLGGRGVTLMVACVSPSAQCLPETLSTLRYASRAQRVTTRPQAPKSPVAKQPQRLETEMLQLQEENRRLQFQLDQMDCKASGLSGARVAWAQRNLYGMLQEFMLENERLRKEKSQLQNSRDLAQNEQRILAQQVHALERRLLSA

相同性解析による交差性が予測される生物種
Full Name kinesin family member 12
Calculated molecular weight 646 aa, 71 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC010626
Gene Symbol KIF12
Gene ID (NCBI) 113220
RRIDAB_2280813
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FN5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIF12(kinesin family member 12) is a member of the kinesin superfamily (KIF), which is a microtubule-dependent ATP-driven molecular motor, and its core is involved in the regulation of organelle transport, cell division and metabolic enzyme degradation. Its functional defects are closely related to bile duct diseases, liver diseases and tumors.

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