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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Eg5 Recombinant monoclonal antibody (84966-2-RR)

    WB analysis using 84966-2-RR (same clone as 84966-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84966-2-RR (Eg5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Eg5 Recombinant monoclonal antibody (84966-2-RR)

    IHC staining of human lung cancer using 84966-2-RR (same clone as 84966-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84966-2-RR (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Recombinant monoclonal antibody (84966-2-RR)

    IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Eg5 Recombinant monoclonal antibody (84966-2-RR)

    IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Eg5 Recombinant monoclonal antibody (84966-2-RR)

    FC experiment of HeLa using 84966-2-RR (same clone as 84966-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Eg5 Recombinant antibody (84966-2-RR, Clone:242611D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84966-2-PBS

    Affinity and Kinetic Characterization of 84966-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84966-2-PBS against Human Eg5 was performed. The affinity constant is 2.69 nM.

  • Affinity and Kinetic Characterization of 84966-2-RR

    Affinity and Kinetic Characterization of 84966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84966-2-RR against Human Eg5 were performed. The affinity constant is 18.3 nM.

Eg5 Recombinant monoclonal antibody, PBS Only

Eg5 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84966-2-PBS
Clone No.242611D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

KIF11, 242611D10, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84966-2-PBS targets Eg5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag20001

Product name: Recombinant human KIF11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 707-1052 aa of BC136474

Sequence: LTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRA

相同性解析による交差性が予測される生物種
Full Name kinesin family member 11
Calculated molecular weight 1056 aa, 119 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC136474
Gene Symbol Eg5
Gene ID (NCBI) 3832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP52732
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

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