Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 23333-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23333-1-AP (Eg5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 23333-1-AP
IP result of anti-Eg5 (IP:23333-1-AP, 4ug; Detection:23333-1-AP 1:1000) with Raji cells lysate 2000ug.
IHC staining of human lung cancer using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human endometrial cancer using 23333-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 23333-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (23333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 23333-1-AP
Immunofluorescent analysis of HepG2 cells using 23333-1-AP (Eg5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23333-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
Positive IP detected in	Raji cells
Positive IHC detected in	human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 17 publications below
IHC	See 9 publications below
IF	See 7 publications below

Product Information

23333-1-AP targets Eg5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18695 Product name: Recombinant human KIF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 707-1052 aa of BC136474 Sequence: LTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRA 相同性解析による交差性が予測される生物種
Full Name	kinesin family member 11
Calculated molecular weight	1056 aa, 119 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	BC136474
Gene Symbol	Eg5
Gene ID (NCBI)	3832
RRID	AB_2716831
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P52732
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Protocols

Product Specific Protocols
IF protocol for Eg5 antibody 23333-1-AP	Download protocol
IHC protocol for Eg5 antibody 23333-1-AP	Download protocol
IP protocol for Eg5 antibody 23333-1-AP	Download protocol
WB protocol for Eg5 antibody 23333-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Br J Cancer Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20 Authors - Iram Fatima View Article
human	IF	Cells Microtubule Association of EML4-ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells Authors - Savvas Papageorgiou View Article
mouse	IHC	Int J Oncol Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway Authors - Ting Xue View Article
human	IHC	Int J Cancer Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma. Authors - Fangqiu Fu View Article
human	WB	Int J Biol Sci KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. Authors - Dang Wei View Article
human	WB	Mol Carcinog Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer. Authors - Benfang Wang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 23333-1-AP

IP experiment of Raji using 23333-1-AP

IHC staining of human lung cancer using 23333-1-AP

IHC staining of human endometrial cancer using 23333-1-AP

IHC staining of human lung cancer using 23333-1-AP

IHC staining of human prostate hyperplasia using 23333-1-AP

IHC staining of human prostate hyperplasia using 23333-1-AP

IHC staining of human endometrial cancer using 23333-1-AP

IF Staining of HepG2 using 23333-1-AP

IF Staining of HepG2 using 23333-1-AP

Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20

Microtubule Association of EML4-ALK V3 Is Key for the Elongated Cell Morphology and Enhanced Migration Observed in V3 Cells

Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.

Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.