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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Polyclonal antibody (31308-1-AP)

    WB analysis using 31308-1-AP (same clone as 31308-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 31308-1-AP (KIAA1715 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Polyclonal antibody (31308-1-AP)

    IHC staining of mouse cerebellum using 31308-1-AP (same clone as 31308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31308-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA1715 Polyclonal antibody (31308-1-AP)

    IF Staining of U2OS using 31308-1-AP (same clone as 31308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA1715 antibody (31308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31308-1-PBS in a different storage buffer formulation.

KIAA1715 Polyclonal antibody, PBS Only

KIAA1715 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31308-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LNP, ER junction formation factor lunapark, ulnaless, Ul, Protein lunapark

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31308-1-PBS targets KIAA1715 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34669

Product name: Recombinant human KIAA1715 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 236-428 aa of BC105132

Sequence: GLHPPGPPLARPILPRERGALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQTNQVIEKASDSEEPEEKQETENEEASVIETNSTVPGADSIPDPELSGESLTAE

相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight51 kDa
GenBank accession numberBC105132
Gene Symbol KIAA1715
Gene ID (NCBI) 80856
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9C0E8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

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