• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30857-1-AP

Synonyms

DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless

Validation Data Gallery

Applications:
  • WB analysis using 30857-1-AP

    WB analysis using 30857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30857-1-AP (KIAA1715 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 30857-1-AP

    IHC staining of mouse cerebellum using 30857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30857-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30857-1-AP targets KIAA1715 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1715 fusion protein Ag33996 相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight53 kDa
GenBank accession numberBC105132
Gene symbol KIAA1715
Gene ID (NCBI) 80856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 30857-1-APDownload protocol
IHC protocol for KIAA1715 antibody 30857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols