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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1199 / CEMIP Polyclonal antibody (31106-1-AP)

    WB analysis using 31106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using KIAA1199 / CEMIP Polyclonal antibody (31106-1-AP)

    IHC staining of human colon cancer using 31106-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 31106-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KIAA1199 / CEMIP Polyclonal antibody (31106-1-AP)

    IF Staining of HeLa using 31106-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KIAA1199 / CEMIP antibody (31106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KIAA1199 / CEMIP Polyclonal antibody

KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31106-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HT-1080 cells
Positive IHC detected inhuman colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31106-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34315

Product name: Recombinant human KIAA1199 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-420 aa of BC020256

Sequence: FERSWGHRGVIVHVIDPKSGTVIHSDRFDTYRSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSSSVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAHPGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFACYDRGRACRSYRVRFLCGKPVRPKLTVTIDTNVN

相同性解析による交差性が予測される生物種
Full Name KIAA1199 / CEMIP
Calculated molecular weight 1361 aa, 153 kDa
Observed molecular weight150 kDa
GenBank accession numberBC020256
Gene Symbol KIAA1199
Gene ID (NCBI) 57214
RRIDAB_3669858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8WUJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Protocols

Product Specific Protocols
IF protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
IHC protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
WB protocol for KIAA1199 / CEMIP antibody 31106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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