Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32467-1-AP targets KIAA1024 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38274 Product name: Recombinant human KIAA1024 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 742-891 aa of NM_015206.2 Sequence: VGLNEEEIKDTGPGDNKDWHRKSKEADRQYDIPPQHRLPKQPKDGFLVEQVFSPHPYPASLKAHMKSNPLYTDMRLTELAEVKRGQPSWTIEEYARNAGDKGKLTALDLQTQESLNPNNLEYWMEDIYTPGYDSLLKRKEAEFRRAKVCK 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1024 |
| Calculated molecular weight | 103kDa，916aa |
| Observed molecular weight | 102 kDa |
| GenBank accession number | NM_015206.2 |
| Gene Symbol | KIAA1024 |
| Gene ID (NCBI) | 23251 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9UPX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA1024 negatively regulates the activities of mTORC1 and mTORC2 by stabilizing the MTOR complex inhibitor DEPTOR, thus inhibiting cell overgrowth. In cultured hippocampal neurons and PC12 cells, knocking down KIAA1024 can activate mTOR signal and promote neurite outgrowth. However, in zebrafish and mouse transplanted tumor models, deletion of this gene will significantly enhance the tumor formation induced by HRAS(G12V), suggesting that it has antitumor effect.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1024 antibody 32467-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |