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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM131L Polyclonal antibody (23360-1-AP)

    WB analysis using 23360-1-AP

    Ramos cells lysates were subjected to SDS PAGE followed by western blot with 23360-1-AP (TMEM131L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM131L Polyclonal antibody (23360-1-AP)

    WB analysis using 23360-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23360-1-AP (TMEM131L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TMEM131L Polyclonal antibody (23360-1-AP)

    IHC staining of mouse colon using 23360-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23360-1-AP (KIAA0922 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KIAA0922 Polyclonal antibody (23360-1-AP)

    IHC staining of mouse kidney using 23360-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23360-1-AP (KIAA0922 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TMEM131L Polyclonal antibody (23360-1-AP)

    IF Staining of A431 using 23360-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KIAA0922 antibody (23360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using TMEM131L Polyclonal antibody (23360-1-AP)

    IF Staining of U-251 using 23360-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using KIAA0922 antibody (23360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TMEM131L Polyclonal antibody

TMEM131L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23360-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0922, Transmembrane protein 131 like, Transmembrane protein 131-like

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRamos cells, HL-60 cells, unboiled HL-60 cells
Positive IHC detected inmouse colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23360-1-AP targets TMEM131L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19898

Product name: Recombinant human KIAA0922 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1110-1461 aa of BC131505

Sequence: WCIQESTREVCKADAEIASSLPAAQREAEGYYQKPEKKCVDKFCSDSSSDCGSSSGSVRASRGSWGSWSSTSSSDGDKKPMVDAQHFLPAGDSVSQNDFPSEAPISLNLSHNICNPMTVNSLPQYAEPSCPSLPAGPTGVEEDKGLYSPGDLWPTPPVCVTSSLNCTLENGVPCVIQESAPVHNSFIDWSATCEGQFSSAYCPLELNDYNAFPEENMNYANGFPCPADVQTDFIDHNSQSTWNTPPNMPAAWGHASFISSPPYLTSTRSLSPMSGLFGSIWAPQSDVYENCCPINPTTEHSTHMENQAVVCKEYYPGFNPFRAYMNLDIWTTTANRNANFPLSRDSSYCGNV

相同性解析による交差性が予測される生物種
Full Name KIAA0922
Calculated molecular weight 1609 aa, 179 kDa
Observed molecular weight 179 kDa
GenBank accession numberBC131505
Gene Symbol KIAA0922
Gene ID (NCBI) 23240
RRIDAB_3085709
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA2VDJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA0922 is also named as TMEM131L (Transmembrane protein 131-like). Human KIAA0922/TMEM131L encodes a transmembrane protein, TMEM131L, that regulates the canonical Wnt/β-catenin signaling pathway by eliciting the lysosome-dependent degradation of phosphorylated LRP6 co-receptor (PMID: 28347816).

Protocols

Product Specific Protocols
IF protocol for TMEM131L antibody 23360-1-APDownload protocol
IHC protocol for TMEM131L antibody 23360-1-APDownload protocol
WB protocol for TMEM131L antibody 23360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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