Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, MOLT-4 cells, SK-BR-3 cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31847-1-AP targets CEP104 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35662 Product name: Recombinant human KIAA0562 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of NM_014704.3 Sequence: MPHKIGFVVVSSSGHEDGFSARELMIHAPTVSGWRSPRFCQFPQEIVLQMVERCRIRKLQLLAHQYMISSKIEFYISESLPEYFAPYQAERFRRLGYVSLCDNEKTGCKARELKSVYVDAVGQFLKLIFHQNHVNKYNIYNQVALVAINIIGDPADFSDESNTASREKLIDHYLGHNSEDPALEGTYARKSDYISPLDDL 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0562 |
| Calculated molecular weight | 104kDa，925aa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | NM_014704.3 |
| Gene Symbol | KIAA0562 |
| Gene ID (NCBI) | 9731 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O60308 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA0562, also known as CEP104 (centrosomal protein 104), is a gene encoding a centrosomal protein that is essential for ciliogenesis and the structural integrity of the cilium tip. During ciliogenesis, CEP104 is translocated from the distal tip of the centriole to the elongated cilium tip. CEP104 is expressed in a variety of cell types, including tissues such as testis and kidney. Mutations in its gene are associated with the autosomal recessive disorder Joubert syndrome, which is characterized by distinctive mid-hindbrain and cerebellar malformations, actinic dyskinesia, irregular breathing, developmental delay, and ataxia. In studies, inhibitors of CEP104 such as Cytochalasin D and Vismodegib have been used to investigate their specific role in cellular function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP104 antibody 31847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |