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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21138-1-AP

Synonyms

C2CD5, CDP138, KIAA0528

Validation Data Gallery

Applications:
  • WB analysis using 21138-1-AP

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21138-1-AP

    WB analysis of HeLa using 21138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21138-1-AP

    WB analysis of HeLa using 21138-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21138-1-AP

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21138-1-AP

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

21138-1-AP targets KIAA0528 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0528 fusion protein Ag15590 相同性解析による交差性が予測される生物種
Full Name KIAA0528
Calculated molecular weight 1000 aa, 110 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC042498
Gene symbol KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols