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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIAA0513 Polyclonal antibody (21478-1-AP)

    IHC staining of human ovary cancer using 21478-1-AP (same clone as 21478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIAA0513 Polyclonal antibody (21478-1-AP)

    IHC staining of human ovary cancer using 21478-1-AP (same clone as 21478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using KIAA0513 Polyclonal antibody (21478-1-AP)

    IHC staining of rat skeletal muscle using 21478-1-AP (same clone as 21478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using KIAA0513 Polyclonal antibody (21478-1-AP)

    IHC staining of rat skeletal muscle using 21478-1-AP (same clone as 21478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIAA0513 Polyclonal antibody (21478-1-AP)

    IF Staining of HepG2 using 21478-1-AP (same clone as 21478-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KIAA0513 antibody (21478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21478-1-PBS in a different storage buffer formulation.

KIAA0513 Polyclonal antibody, PBS Only

KIAA0513 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 21478-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Uncharacterized protein KIAA0513

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21478-1-PBS targets KIAA0513 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15624

Product name: Recombinant human KIAA0513 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-301 aa of BC030280

Sequence: METPEVPVGSLIDFGPEAPTSSPLEAPPPVLQDGDGSLGDGASESETTESADSENDMGESPSHPSWDQDRRSSSNESFSSNQSTESTQDEETLALRDFMRGYVEKIFSGGEDLDQEEKAKFGEYCSSENGKGREWFARYVSAQRCNSKCVSEATFYRLVQSFAVVLFECHQMDDFGPAKNLMTMCFTYYHIGKPQLLPPESREKPAGSIDSYLKSANSWLAEKKDIAERLLKNTSARTENVKGFFGGLETKLKGPLARRNEEDENKPQEKRPRAVTAYSPEDEKKGEKIYLYTHLKQQPIW

相同性解析による交差性が予測される生物種
Full Name KIAA0513
Calculated molecular weight 411 aa, 47 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC030280
Gene Symbol KIAA0513
Gene ID (NCBI) 9764
RRIDAB_10755300
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60268
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.

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