Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MG-63 cells, U2OS cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34075-1-AP targets AP5Z1/SPG48 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26954 Product name: Recombinant human KIAA0415 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-364 aa of BC037399 Sequence: TLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASV 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0415 |
| Calculated molecular weight | 807 aa, 88 kDa |
| Observed molecular weight | 88 kDa |
| GenBank accession number | BC037399 |
| Gene Symbol | AP5Z1/SPG48 |
| Gene ID (NCBI) | 9907 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O43299 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for AP5Z1/SPG48 antibody 34075-1-AP | Download protocol |
| WB protocol for AP5Z1/SPG48 antibody 34075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |