Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
25684-1-AP targets KIAA0368 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22309 Product name: Recombinant human KIAA0368 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1501-1845 aa of BC021127 Sequence: EECNLWTEVWQENVPGSFGGIRLYLQELITITQKALQSQSWKMKAQGAIAMASIAKQTSSLVPPYLGMILTALLQGLAGRTWAGKEELLKAIACVVTACSAELEKSVPNQPSTNEILQAVLKECSKENVKYKIVAISCAADILKATKEDRFQEFSNIVIPLIKKNSLESSGVRTTKNEEENEKEKELQLEYLLGAFESLGKAWPRNAETQRCYRQELCKLMCERLKLSTWKVQLGVLQSMNAFFQGLMLLEEEHADPEALAEILLETCKSITYSLENKTYSSVRTEALSVIELLLKKLEESKQWECLTSECRVLLIESLATMEPDSRPELQEKAALLKKTLENLE 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0368 |
| Calculated molecular weight | 1845 aa, 204 kDa |
| Observed molecular weight | 205 kDa |
| GenBank accession number | BC021127 |
| Gene Symbol | KIAA0368 |
| Gene ID (NCBI) | 23392 |
| RRID | AB_2880193 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VYK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ecm29 binds specifically to 26 S proteasomes. Ecm29 has been reported to stabilize proteasomes, to remodel proteasomes upon stress, to be involved in membrane-associated localization of proteasomes, and, more recently, to be involved in quality control or assembly of the proteasome. Ecm29 acts as a proteasome inhibitor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA0368 antibody 25684-1-AP | Download protocol |
| IHC protocol for KIAA0368 antibody 25684-1-AP | Download protocol |
| IF protocol for KIAA0368 antibody 25684-1-AP | Download protocol |
| IP protocol for KIAA0368 antibody 25684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |