Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32028-1-AP targets KIAA0196 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36916 Product name: Recombinant human KIAA0196 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 950-1159 aa of NM_014846.3 Sequence: KVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0196 |
| Calculated molecular weight | 134kDa，1159aa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | NM_014846.3 |
| Gene Symbol | KIAA0196 |
| Gene ID (NCBI) | 9897 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q12768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA0196 is a core component of the WASH complex, an actin-regulating complex involved in intracellular trafficking, mitochondrial metabolism, and myelination. It plays a crucial role in maintaining the structural integrity of myofibrils and regulating cardiac function. Recent studies have identified KIAA0196 as a susceptibility gene for abnormal cardiac development. Mutations or reduced expression of KIAA0196 can lead to cardiac defects, such as enlarged hearts and myocardial contractile dysfunction (PMID: 32417190).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA0196 antibody 32028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |