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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA0182 Polyclonal antibody (24947-1-AP)

    WB analysis using 24947-1-AP (same clone as 24947-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24947-1-AP (KIAA0182 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of human kidney using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KIAA0182 Polyclonal antibody (24947-1-AP)

    IHC staining of mouse testis using 24947-1-AP (same clone as 24947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KIAA0182 Polyclonal antibody (24947-1-AP)

    IF Staining of U2OS using 24947-1-AP (same clone as 24947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA0182 antibody (24947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24947-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KIAA0182 Polyclonal antibody, PBS Only

KIAA0182 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24947-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24947-1-PBS targets KIAA0182 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20674

Product name: Recombinant human KIAA0182 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 764-1113 aa of BC037556

Sequence: TIFNLTHISAEKRKDKERLVEMLRAMKQKALSAAVADSLTNSPRDSPAVSLSEPATQQASLDVEKPVGAAASLSDIPKAAEPGKLEQVRPQELSRVQELAPASGEKARLSEAPGGKKSLSMLHYIRGAAPKDIPVPLSHSTNGKSKPWEPFVAEEFAHQFHESVLQSTQKALQKHKGSVAVLSAEQNHKVDTSVHYNIPELQSSSRAPPPQHNGQQEPPTARKGPPTQELDRDSEEEEEEDDEDGEDEEEVPKRKWQGIEAVFEAYQEHIEEQNLERQVLQTQCRRLEARHYSLSLTAEQLSHSVAELRSQKQKMVSERERLQAELDHLRKCLALPAMHWPRGYLKGYPR

相同性解析による交差性が予測される生物種
Full Name KIAA0182
Calculated molecular weight 1217 aa, 136 kDa
Observed molecular weight 136 kDa
GenBank accession numberBC037556
Gene Symbol KIAA0182
Gene ID (NCBI) 23199
RRIDAB_2879815
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14687
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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