Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, MG-63 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20537-1-AP targets PAF15 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14522 Product name: Recombinant human KIAA0101 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-111 aa of BC005832 Sequence: MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0101 |
| Calculated molecular weight | 111 aa, 12 kDa |
| Observed molecular weight | 15-17 kDa |
| GenBank accession number | BC005832 |
| Gene Symbol | KIAA0101 |
| Gene ID (NCBI) | 9768 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAF15, also known as PCLAF/KIAA0101, located at 15q14, is a small nuclear protein that binds to PCNA. Its expression fluctuated with the cell cycle, with high expression in S/G2 phase and degradation in G1 phase. Anchoring replication fork by PIP motif, specifically stabilizing the synthesis of lag chain, and regulating S-phase checkpoint; Participate in cross-injury repair when DNA is damaged. The gene is abnormally overexpressed in various solid tumors, which drives tumor progress by disturbing genome stability, promoting proliferation and antagonizing apoptosis. The overexpression indicates poor prognosis, and it is a tumor prognosis marker and potential therapeutic target. There are reports of 15kDa bands and 25 kDa bands with ubiquitination modification (PMID: 41606318).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAF15 antibody 20537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |