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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PUF-A,KIAA0020 Polyclonal antibody (25491-1-AP)

    WB analysis of A549 using 25491-1-AP

    WB result of KIAA0020 antibody (25491-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIAA0020 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using PUF-A,KIAA0020 Polyclonal antibody (25491-1-AP)

    WB analysis using 25491-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brown disease using PUF-A,KIAA0020 Polyclonal antibody (25491-1-AP)

    IHC staining of human brown disease using 25491-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brown disease using PUF-A,KIAA0020 Polyclonal antibody (25491-1-AP)

    IHC staining of human brown disease using 25491-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 25491-1-AP (PUF-A,KIAA0020 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

PUF-A,KIAA0020 Polyclonal antibody

PUF-A,KIAA0020 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25491-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KIAA0020, PUM3, Minor histocompatibility antigen HA-8, HLA-HA8, HBV X-transactivated gene 5 protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25491-1-AP targets PUF-A,KIAA0020 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22011

Product name: Recombinant human KIAA0020 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 283-648 aa of BC016137

Sequence: YKSADHPTLDKVLELQPEKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSEMIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKDTEVRRRELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGIEILLEKLST

相同性解析による交差性が予測される生物種
Full Name KIAA0020
Calculated molecular weight 648 aa, 74 kDa
Observed molecular weight 69-70 kDa
GenBank accession numberBC016137
Gene Symbol PUF-A/KIAA0020
Gene ID (NCBI) 9933
RRIDAB_2918090
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PUF-A,KIAA0020 antibody 25491-1-APDownload protocol
WB protocol for PUF-A,KIAA0020 antibody 25491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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