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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KHSRP Recombinant antibody (82931-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82931-5-RR (KHSRP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KHSRP Recombinant antibody (82931-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82931-5-RR (KHSRP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KHSRP Recombinant antibody (82931-5-RR)

    IF Staining of HeLa using 82931-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-5-RR, Clone: 230189A2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using KHSRP Recombinant antibody (82931-5-RR)

    FC experiment of HeLa using 82931-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug KHSRP Recombinant antibody (82931-5-RR, Clone:230189A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82931-5-RR

    Affinity and Kinetic Characterization of 82931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-5-RR against Human KHSRP were performed. The affinity constant is 2.11 nM.

KHSRP Recombinant monoclonal antibody

KHSRP Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82931-5-RR
Clone No.230189A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82931-5-RR targets KHSRP in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33890

Product name: Recombinant human KHSRP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-710 aa of NM_003685

Sequence: APPAQGEPPQPPPTGQSDYTKAWEEYYKKIGQQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQPPPTQQGQQQAQ

相同性解析による交差性が予測される生物種
Full Name KH-type splicing regulatory protein
Calculated molecular weight73 kDa
GenBank accession numberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670672
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

Protocols

Product Specific Protocols
FC protocol for KHSRP antibody 82931-5-RRDownload protocol
IF protocol for KHSRP antibody 82931-5-RRDownload protocol
IHC protocol for KHSRP antibody 82931-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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