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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KHK Recombinant monoclonal antibody (85386-1-RR)

    WB analysis using 85386-1-RR (same clone as 85386-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85386-1-RR (KHK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KHK Recombinant monoclonal antibody (85386-1-RR)

    WB analysis of HepG2 using 85386-1-RR (same clone as 85386-1-PBS)

    WB result of KHK antibody (85386-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHK transfected HepG2 cells. This data was developed using the same antibody clone with 85386-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85386-1-RR

    Affinity and Kinetic Characterization of 85386-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85386-1-RR against Human KHK were performed. The affinity constant is 8.46 nM.

KHK Recombinant monoclonal antibody, PBS Only

KHK Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85386-1-PBS
Clone No.242745D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

242745D5, EC:2.7.1.3, Ketohexokinase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85386-1-PBS targets KHK in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8182

Product name: Recombinant human KHK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC006233

Sequence: MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTILSLLGAPCAFMGSMAPGHVADFVLDDLRRYSVDLRYTVFQTTGSVPIATVIINEASGSRTILYYDRSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

相同性解析による交差性が予測される生物種
Full Name ketohexokinase (fructokinase)
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC006233
Gene Symbol KHK
Gene ID (NCBI) 3795
RRIDAB_3744181
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP50053
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.

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