Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, human liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
Product Information
15681-1-AP targets KHK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8182 Product name: Recombinant human KHK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC006233 Sequence: MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTILSLLGAPCAFMGSMAPGHVADFVLDDLRRYSVDLRYTVFQTTGSVPIATVIINEASGSRTILYYDRSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV 相同性解析による交差性が予測される生物種 |
| Full Name | ketohexokinase (fructokinase) |
| Calculated molecular weight | 298 aa, 33 kDa |
| Observed molecular weight | 33-35 kDa |
| GenBank accession number | BC006233 |
| Gene Symbol | KHK |
| Gene ID (NCBI) | 3795 |
| RRID | AB_2130496 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50053 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KHK antibody 15681-1-AP | Download protocol |
| IHC protocol for KHK antibody 15681-1-AP | Download protocol |
| IP protocol for KHK antibody 15681-1-AP | Download protocol |
| WB protocol for KHK antibody 15681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Adv Sci (Weinh) NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer | ||
Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. | ||
Cancer Sci Identification of TPI1 As a potential therapeutic target in pancreatic cancer with dependency of TP53 mutation using multi-omics analysis | ||
Endocrinology Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance | ||
Biochem Biophys Res Commun Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma. |
