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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HUVEC cells using VEGFR2/CD309 Recombinant antibody (83049-4-RR)

    FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

    1x10^6 HUVEC cells were surface stained with 0.25 ug VEGFR2/CD309 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83049-4-RR

    Affinity and Kinetic Characterization of 83049-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

VEGFR2/CD309 Recombinant monoclonal antibody, PBS Only

VEGFR2/CD309 Uni-rAb® Recombinant Antibody for WB, IHC, FC, Indirect ELISA
Cat No. 83049-4-PBS
Clone No.230238D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, Indirect ELISA

Synonyms

KDR, VEGFR2, VEGFR2/KDR, 230238D7, CD309

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83049-4-PBS targets VEGFR2/CD309 in WB, IHC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0218

Product name: Recombinant Human VEGFR2/KDR protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 20-764 aa of NM_002253

Sequence: ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKTNLE

相同性解析による交差性が予測される生物種
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated molecular weight 152 kDa
Observed molecular weight150 kDa, 230 kDa
GenBank accession numberNM_002253
Gene Symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35968-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

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