Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, human placenta tissue, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
19915-1-AP targets KDM3B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13795 Product name: Recombinant human KDM3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1127-1382 aa of BC001202 Sequence: SRQNKSVLRPAVTNGMSQLPSINPSASSGNETTFSGGGGPAPVTTPEPDHVPKADSTDIRSEEPLKTDSSASNSNSELKAIRPPCPDTAPPSSALHWLADLATQKAKEETKEAGSLRSVLNKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPTASNNKTEGSSLRDLLHSGPGKLPQTPLDTGIPFPPVFSTSSAGVKSKASLPNFLDHIIASVVENKKTSDASKRACNLTDTQKEVKEMVMGLNVLDPHTSHS 相同性解析による交差性が予測される生物種 |
| Full Name | lysine (K)-specific demethylase 3B |
| Calculated molecular weight | 1761 aa, 192 kDa |
| Observed molecular weight | 192 kDa, 85 kDa |
| GenBank accession number | BC001202 |
| Gene Symbol | KDM3B |
| Gene ID (NCBI) | 51780 |
| RRID | AB_10693529 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7LBC6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM3B antibody 19915-1-AP | Download protocol |
| IF protocol for KDM3B antibody 19915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. | ||
eLife SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing |