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Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    WB analysis of human cerebellum using 14212-1-AP (same clone as 14212-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    WB analysis of human brain using 14212-1-AP (same clone as 14212-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of fetal human brain tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IP experiment of fetal human brain using 14212-1-AP (same clone as 14212-1-PBS)

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KChIP1 Polyclonal antibody (14212-1-AP)

    IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using KChIP1 Polyclonal antibody (14212-1-AP)

    FC experiment of HepG2 using 14212-1-AP (same clone as 14212-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KChIP1 Polyclonal antibody (14212-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

KChIP1 Polyclonal antibody, PBS Only

KChIP1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 14212-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14212-1-PBS targets KChIP1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5432

Product name: Recombinant human KCNIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC050375

Sequence: MGAVMGTFSSLQTKQRRPSKDKIEDELEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEDTFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM

相同性解析による交差性が予測される生物種
Full Name Kv channel interacting protein 1
Calculated molecular weight 227 aa, 27 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC050375
Gene Symbol KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZI2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

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