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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCTD7 Polyclonal antibody (32488-1-AP)

    WB analysis using 32488-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32488-1-AP (KCTD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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KCTD7 Polyclonal antibody

KCTD7 Polyclonal Antibody for WB, ELISA
Cat No. 32488-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BTB/POZ domain-containing protein KCTD7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32488-1-AP targets KCTD7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCTD7 fusion protein Ag35756 相同性解析による交差性が予測される生物種
Full Name potassium channel tetramerisation domain containing 7
Calculated molecular weight33 kDa，289 aa
Observed molecular weight62 kDa
GenBank accession numberNM_153033.4
Gene Symbol KCTD7
Gene ID (NCBI) 154881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96MP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCTD7, or Potassium Channel Tetramerization Domain Containing 7, is a member of the KCTD family of proteins. The role of KCTD7 in regulating autophagy-lysosome pathways, which are essential for cellular homeostasis and response to nutrient stress (PMID: 36964131). It has been implicated in several neurodegenerative disorders, particularly progressive myoclonic epilepsy (PME) and neuronal ceroid lipofuscinosis (NCL). The deficiency of KCTD7 results in the accumulation of lipofuscin and lipid droplets, which are hallmarks of NCL (PMID: 33970744). The 62 kDa form most likely corresponds to an SDS-stable dimer (PMID: 22748208).

Protocols

Product Specific Protocols
WB protocol for KCTD7 antibody 32488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols