Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32898-1-AP targets KCTD4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37112 Product name: Recombinant human KCTD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-259 aa of BC018063 Sequence: MERKINRREKEKEYEGKHNSLEDTDQGKNCKSTLMTLNVGGYLYITQKQTLTKYPDTFLEGIVNGKILCPFDADGHYFIDRDGLLFRHVLNFLRNGELLLPEGFRENQLLAQEAEFFQLKGLAEEVKSRWEKEQLTPRETTFLEITDNHDRSQGLRIFCNAPDFISKIKSRIVLVSKSRLDGFPEEFSISSNIIRFKYFIKSENGTRLVLKEDNTFVCTLETLKFEAIMMALKCGFRLLTSLDCSKGSIVHSDALHFIK 相同性解析による交差性が予測される生物種 |
| Full Name | potassium channel tetramerisation domain containing 4 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC018063 |
| Gene Symbol | KCTD4 |
| Gene ID (NCBI) | 386618 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WVF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The family of potassium channel tetramerization domain (KCTD) proteins consists of 26 members (PMID: 24268103). Potassium channel tetramerization domain containing 4 (KCTD4) is a protein that interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer. KCTD4 expression is upregulated in metastatic esophageal squamous cell carcinoma (ESCC), and high KCTD4 expression is associated with poor prognosis in patients with ESCC. This process activates fibroblasts in a paracrine manner, promoting cancer cell invasion via MMP24 signaling as positive feedback (PMID: 37799381).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCTD4 antibody 32898-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |