Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 15523-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15523-1-AP
Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15523-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15523-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15523-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15523-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15523-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human bowen disease using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15523-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 15523-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

KCTD12 Polyclonal antibody

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15523-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, human brain tissue, HEK-293 cells, Hela cells, rat brain tissue
Positive IHC detected in	human bowen disease, human intrahepatic cholangiocarcinoma tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

15523-1-AP targets KCTD12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5716 Product name: Recombinant human KCTD12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-325 aa of BC013764 Sequence: MALADSTRGLPNGGGGGGGSGSSSSSAEPPLFPDIVELNVGGQVYVTRRCTVVSVPDSLLWRMFTQQQPQELARDSKGRFFLDRDGFLFRYILDYLRDLQLVLPDYFPERSRLQREAEYFELPELVRRLGAPQQPGPGPPPSRRGVHKEGSLGDELLPLGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYITIGYRGSYTIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKFNFLEQAFDKLSESGFHMVACSSTGTCAFASSTDQSEDKIWTSYTEYVFCRE 相同性解析による交差性が予測される生物種
Full Name	potassium channel tetramerisation domain containing 12
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC013764
Gene Symbol	KCTD12
Gene ID (NCBI)	115207
RRID	AB_2132006
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96CX2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KCTD12 also known as PFET1 or PFETIN, which contains a voltage-gated potassium (K+) channel tetramerization T1 domain and a BTB/POZ (Bric-a-brac, Tram-track, Broad complex poxvirus and zinc finger) domain, belongs to the KCTD family and was initially identified in cochlea. In addition to being a K+ channel protein that responds to membrane potential7, KCTD12 also acts as an auxiliary subunit of GABAB (γ-aminobutyric acid type B) receptors, which regulate emotionality and neuronal excitability.

Protocols

Product Specific Protocols
IF protocol for KCTD12 antibody 15523-1-AP	Download protocol
IHC protocol for KCTD12 antibody 15523-1-AP	Download protocol
WB protocol for KCTD12 antibody 15523-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Death Dis Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis Authors - Lifu Luo View Article
human	WB,IHC,IF	Oncogene KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Authors - Y Zhong KD Validated View Article
mouse	WB,IF	Pharmacol Res The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice. Authors - Si-Long Deng View Article
mouse	WB,IF	J Neurosci KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in Habenula Cholinergic Neurons. Authors - Yuqi Ren KO Validated View Article
human	WB,IHC	Sci Rep KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway. Authors - Liping Li KD Validated View Article
mouse	IF	Front Mol Neurosci The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS. Authors - Javier H Jara View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 15523-1-AP

WB analysis of HeLa using 15523-1-AP

WB analysis of rat brain using 15523-1-AP

WB analysis of mouse brain using 15523-1-AP

WB analysis of MCF-7 using 15523-1-AP

WB analysis of human brain using 15523-1-AP

WB analysis of HEK-293 using 15523-1-AP

IHC staining of human bowen disease using 15523-1-AP

IHC staining of human bowen disease using 15523-1-AP

IHC staining of human stomach using 15523-1-AP

IHC staining of human stomach using 15523-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

IF Staining of HeLa using 15523-1-AP

IF Staining of MCF-7 using 15523-1-AP

KCTD12 Polyclonal antibody

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis

KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.

The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice.

KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in Habenula Cholinergic Neurons.

KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway.

The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS.