Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HuH-7 cells, Jurkat cells, K-562 cells, MCF-7 cells, U2OS cells |
| Positive IP detected in | HuH-7 cells |
| Positive IHC detected in | human breast cancer tissue, human intrahepatic cholangiocarcinoma tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
Product Information
27279-1-AP targets KCTD10 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KCTD10 fusion protein Ag25971 相同性解析による交差性が予測される生物種 |
| Full Name | potassium channel tetramerisation domain containing 10 |
| Calculated molecular weight | 313 aa, 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC040062 |
| Gene Symbol | KCTD10 |
| Gene ID (NCBI) | 83892 |
| RRID | AB_2880827 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3F6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| IHC protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| IP protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.
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Front Cell Dev Biol Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.
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Genet Res (Camb) Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis
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