• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNS2 Polyclonal antibody

KCNS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13443-1-AP

Synonyms

KCNS2, KIAA1144, KV9.2

Validation Data Gallery

Applications:
  • WB analysis of Y79 using 13443-1-AP

    WB analysis of Y79 using 13443-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 13443-1-AP

    WB analysis of HepG2 using 13443-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 13443-1-AP

    WB analysis of mouse brain using 13443-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13443-1-AP (KCNS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 13443-1-AP

    IP experiment of HepG2 using 13443-1-AP

    IP result of anti-KCNS2 (IP:13443-1-AP, 4ug; Detection:13443-1-AP 1:300) with HepG2 cells lysate 3400ug.

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human eye using 13443-1-AP

    IHC staining of human eye using 13443-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 13443-1-AP (KCNS2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inY79 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13443-1-AP targets KCNS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNS2 fusion protein Ag4271 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC027932
Gene symbol KCNS2
Gene ID (NCBI) 3788
RRIDAB_2249596
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCNS2 antibody 13443-1-APDownload protocol
IHC protocol for KCNS2 antibody 13443-1-APDownload protocol
IP protocol for KCNS2 antibody 13443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols