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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNQ5 Polyclonal antibody (28000-1-AP)

    WB analysis using 28000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28000-1-AP (KCNQ5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using KCNQ5 Polyclonal antibody (28000-1-AP)

    IP experiment of Raji using 28000-1-AP

    IP result of anti-KCNQ5 (IP:28000-1-AP, 4ug; Detection:28000-1-AP 1:500) with Raji cells lysate 1600 ug.

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KCNQ5 Polyclonal antibody

KCNQ5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 28000-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalu-1 cells, HEL cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IP detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28000-1-AP targets KCNQ5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNQ5 fusion protein Ag27622 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, KQT-like subfamily, member 5
Calculated molecular weight 102 kDa
Observed molecular weight100 kDa
GenBank accession numberNM_001160130
Gene Symbol KCNQ5
Gene ID (NCBI) 56479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Potassium voltage-gated channel subfamily Q member 5 (KCNQ5, also known as Kv7.5 and MRD46) is widely expressed in the brain and generates M-type current. It has a role in the regulation of neuronal excitability (PMID: 10816588). KCNQ5 mutations are associated with increased excitability and decreased repolarization reserve, lead to pathophysiology (PMID: 28669405).

Protocols

Product Specific Protocols
WB protocol for KCNQ5 antibody 28000-1-APDownload protocol
IP protocol for KCNQ5 antibody 28000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols