Validation Data Gallery
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Tested Applications
| Positive WB detected in | fetal human brain tissue, human brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5618 Product name: Recombinant human KCNMB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 46-176 aa of BC042446 Sequence: DLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEHQLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLW 相同性解析による交差性が予測される生物種 |
| Full Name | potassium large conductance calcium-activated channel, subfamily M, beta member 4 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC042446 |
| Gene Symbol | KCNMB4 |
| Gene ID (NCBI) | 27345 |
| RRID | AB_2280832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86W47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCNMB4 antibody 60122-1-Ig | Download protocol |
| WB protocol for KCNMB4 antibody 60122-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells. | ||
Mitochondrion Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium | ||
Peptides Apelin/APJ increased renal blood flow through endothelial BKCa channel induced p-eNOS and ET-1 in diabetic conditions |
