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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNMA1 Polyclonal antibody (22158-1-AP)

    WB analysis using 22158-1-AP (same clone as 22158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of rat brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of rat brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of mouse cerebellum using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IHC staining of mouse cerebellum using 22158-1-AP (same clone as 22158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IF Staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNMA1 Polyclonal antibody (22158-1-AP)

    IF Staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

KCNMA1 Polyclonal antibody, PBS Only

KCNMA1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 22158-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

BK channel, BKCA alpha, BKTM, hSlo, K(VCA)alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22158-1-PBS targets KCNMA1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17802

Product name: Recombinant human KCNMA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 829-1178 aa of BC137115

Sequence: KREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQKYVQEERL

相同性解析による交差性が予測される生物種
Full Name potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Calculated molecular weight 1236 aa, 138 kDa
Observed molecular weight~130 kDa
GenBank accession numberBC137115
Gene Symbol KCNMA1
Gene ID (NCBI) 3778
RRIDAB_3669389
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12791
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.

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