Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
14136-1-AP targets KCNK5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5355 Product name: Recombinant human KCNK5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 244-499 aa of BC060793 Sequence: WKVSMFVEVHKAIKKRRRRRKESFESSPHSRKALQVKGSTASKDVNIFSFLSKKEETYNDLIKQIGKKAMKTSGGGETGPGPGLGPQGGGLPALPPSLVPLVVYSKNRVPTLEEVSQTLRSKGHVSRSPDEEAVARAPEDSSPAPEVFMNQLDRISEECEPWDAQDYHPLIFQDASITFVNTEAGLSDEETSKSSLEDNLAGEESPQQGAEAKAPLNMGEFPSSSESTFTSTESELSVPYEQLMNEYNKANSPKGT 相同性解析による交差性が予測される生物種 |
| Full Name | potassium channel, subfamily K, member 5 |
| Calculated molecular weight | 499 aa, 55 kDa |
| Observed molecular weight | 70 kDa, 55 kDa |
| GenBank accession number | BC060793 |
| Gene Symbol | KCNK5 |
| Gene ID (NCBI) | 8645 |
| RRID | AB_2280811 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Potassium channel subfamily K member 5 (KCNK5, also termed TASK2) is a potassium channel mediating voltage-dependent outward-rectifying currents in response to membrane depolarization. Its voltage sensing is coupled to the potassium electrochemical gradient via an "ion flux gating" mechanism, in which gate opening is triggered by outward rather than inward ion movement (PMID: 26919430; 36063992; 9812978). Immunoblotting analysis of KCNK5 detects two protein bands at 55 kDa and 70 kDa, representing the monomeric and glycosylated forms of the KCNK5 potassium channel, respectively (PMID: 24285684; 26140335).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNK5 antibody 14136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |