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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNK4 Polyclonal antibody

KCNK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27113-1-AP

Synonyms

K2p4.1, KCNK4, TRAAK, TRAAK1, Two pore K(+) channel KT4.1

Validation Data Gallery

Applications:
  • WB analysis using 27113-1-AP

    WB analysis using 27113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27113-1-AP (KCNK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse eye using 27113-1-AP

    WB analysis of mouse eye using 27113-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 27113-1-AP (KCNK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27113-1-AP

    IHC staining of human brain using 27113-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 27113-1-AP

    IHC staining of human brain using 27113-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 27113-1-AP is KCNK4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNK4 fusion protein Ag25931 相同性解析による交差性が予測される生物種
Full Name potassium channel, subfamily K, member 4
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC033577
Gene symbol KCNK4
Gene ID (NCBI) 50801
RRIDAB_2880761
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCNK4 antibody 27113-1-APDownload protocol
IHC protocol for KCNK4 antibody 27113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Funct Integr Genomics

LncRNA SLC25A21-AS1 increases the chemosensitivity and inhibits the progression of ovarian cancer by upregulating the expression of KCNK4

Authors - Ke Huang
View Article