Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IHC | See 3 publications below |
Product Information
19965-1-AP targets Kir2.1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | potassium inwardly-rectifying channel, subfamily J, member 2 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 50 kDa, 60 kDa |
| GenBank accession number | NM_000891 |
| Gene Symbol | Kir2.1 |
| Gene ID (NCBI) | 3759 |
| RRID | AB_10859827 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P63252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KCNJ2, also named as HHBIRK1, HHIRK1, IRK1, KIR2.1, LQT7 and SQT3, belongs to the inward rectifier-type potassium channel family. KCNJ2 probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ2 can be blocked by extracellular barium or cesium. Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7). Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3). The antibody recognizes the C-term of KCNJ2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Kir2.1 antibody 19965-1-AP | Download protocol |
| WB protocol for Kir2.1 antibody 19965-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles 124I-labelled BMSC-Derived Extracellular Vesicles Deliver CRISPR/Cas9 Ribonucleoproteins With a GFP-Reporter System to Inhibit Osteosarcoma Proliferation and Metastasis | ||
Nat Commun Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters. | ||
J Adv Res Mitochondrial dysfunction drives ZBP1-mediated PANoptosis to increase the susceptibility of heart failure with preserved ejection fraction-associated atrial fibrillation. | ||
Cell Commun Signal KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma | ||
Phytomedicine Divaroside alleviates barium chloride-induced arrhythmia by activating the Nrf2/HO-1 axis to modulate autophagy and calcium homeostasis. | ||
Sci China Life Sci m6A reader YTHDF2 governs the onset of atrial fibrillation by modulating Cacna1c translation |