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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNJ18 Recombinant antibody (84203-5-RR)

    WB analysis using 84203-5-RR (same clone as 84203-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84203-5-RR (KCNJ18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using KCNJ18 Recombinant antibody (84203-5-RR)

    IHC staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84203-5-RR (KCNJ18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using KCNJ18 Recombinant antibody (84203-5-RR)

    IHC staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84203-5-RR (KCNJ18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using KCNJ18 Recombinant antibody (84203-5-RR)

    IF Staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (84203-5-RR, Clone: 241381A2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84203-5-RR

    Affinity and Kinetic Characterization of 84203-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84203-5-RR against Human KCNJ18 were performed. The affinity constant is below 3.06 nM.

KCNJ18 Recombinant monoclonal antibody, PBS Only

KCNJ18 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84203-5-PBS
Clone No.241381A2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TTPP2, KIR2.6, Inward rectifier potassium channel 18, 241381A2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84203-5-PBS targets KCNJ18 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34177

Product name: Recombinant human KCNJ18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-433 aa of NM_001194958

Sequence: STPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRGSEI

相同性解析による交差性が予測される生物種
Full Name similar to hkir2.2x
Calculated molecular weight 49 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_001194958
Gene Symbol KCNJ18
Gene ID (NCBI) 100134444
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDB7U540
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

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