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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using KCNJ18 Polyclonal antibody (30911-1-AP)

    WB analysis of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 30911-1-AP (KCNJ18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30911-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using KCNJ18 Polyclonal antibody (30911-1-AP)

    IF Staining of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (30911-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30911-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skin tissue using KCNJ18 Polyclonal antibody (30911-1-AP)

    IF Staining of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (30911-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30911-1-PBS in a different storage buffer formulation.

KCNJ18 Polyclonal antibody, PBS Only

KCNJ18 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 30911-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30911-1-PBS targets KCNJ18 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34177

Product name: Recombinant human KCNJ18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-433 aa of NM_001194958

Sequence: STPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRGSEI

相同性解析による交差性が予測される生物種
Full Name similar to hkir2.2x
Calculated molecular weight 49 kDa
Observed molecular weight45 kDa
GenBank accession numberNM_001194958
Gene Symbol KCNJ18
Gene ID (NCBI) 100134444
RRIDAB_3669782
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDB7U540
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

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