Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30853-1-AP targets KCNH2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34230 Product name: Recombinant human KCNH2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 880-1159 aa of NM_000238 Sequence: GFSRQRKRKLSFRRRTDKDTEQPGEVSALGPGRAGAGPSSRGRPGGPWGESPSSGPSSPESSEDEGPGRSSSPLRLVPFSSPRPPGEPPGGEPLMEDCEKSSDTCNPLSGAFSGVSNIFSFWGDSRGRQYQELPRCPAPTPSLLNIPLSSPGRRPRGDVESRLDALQRQLNRLETRLSADMATVLQLLQRQMTLVPPAYSAVTTPGPGPTSTSPLLPVSPLPTLTLDSLSQVSQFMACEELPPGAPELPQEGPTRRLSLPGQLGALTSQPLHRHGSDPGS 相同性解析による交差性が予測される生物種 |
| Full Name | potassium voltage-gated channel, subfamily H (eag-related), member 2 |
| Calculated molecular weight | 127 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | NM_000238 |
| Gene Symbol | KCNH2 |
| Gene ID (NCBI) | 3757 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q12809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Voltage-gated inwardly rectifying potassium channel KCNH2 (also known as ERG1, Kv11.1, and HERG) is a key member of the voltage-gated potassium (Kv) channel family (PMID: 16382104). Growing evidence has indicated that the association between KCNH2 and hypoglycemia, an adverse effect induced by KCNH2 blockers, implies a potential regulatory role of this channel in the maintenance of blood glucose homeostasis (PMID: 21224236; 31863282). Mutations in the KCNH2 gene are recognized as one of the etiological factors of congenital long QT syndrome (LQTS), a rare syndrome that carries an increased risk of cardiac arrhythmias, including the polymorphic ventricular tachycardia termed torsades de pointes (TdP) (PMID: 17143043; 16554806).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNH2 antibody 30853-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |