Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse pancreas tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17920-1-AP targets KCNE2 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12336 Product name: Recombinant human KCNE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC093892 Sequence: MSTLSNFTQTLEDVFRRIFITYMDNWRQNTTAEQEALQAKVDAENFYYVILYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQYIVEDWQEKYKSQILNLEESKATIHENIGAAGFKMSP 相同性解析による交差性が予測される生物種 |
| Full Name | potassium voltage-gated channel, Isk-related family, member 2 |
| Calculated molecular weight | 123 aa, 14 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC093892 |
| Gene Symbol | KCNE2 |
| Gene ID (NCBI) | 9992 |
| RRID | AB_3669283 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6J6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Potassium voltage-gated channel subfamily E regulatory subunit 2 (KCNE2, also known as LQT5, LQT6, ATFB4, and MIRP1) functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta subunits (PMID: 10219239; 11034315; 11101505; 12185453; 20533308). KCNE2 beta subunit modulates the gating kinetics and enhances the stability of the channel complex (PMID: 10219239; 11034315; 11101505; 12185453; 20533308). It is expressed in various parts of the heart, with higher levels observed in the ventricles and Purkinje tissues compared to the atria (PMID: 39272981). KCNE2 has three forms, such as the core (15 kDa), singly glycosylated (20 kDa), and doubly glycosylated (25 kDa) forms (PMID: 15066947).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNE2 antibody 17920-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |