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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNAB1 Polyclonal antibody

KCNAB1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14697-1-AP

Synonyms

AKR6A3, hKvb3, hKvBeta3, K(+) channel subunit beta 1, KCNA1B, KCNAB1, KV BETA 1, Kvb1.3

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 14697-1-AP

    WB analysis of mouse heart using 14697-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 14697-1-AP

    WB analysis of Neuro-2a using 14697-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 14697-1-AP

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of Hela cells, using KCNAB1 antibody 14697-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HeLa using 14697-1-AP

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14697-1-AP (KCNAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue, Neuro-2a cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1500
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14697-1-AP targets KCNAB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNAB1 fusion protein Ag6339 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, shaker-related subfamily, beta member 1
Calculated molecular weight 47 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC043166
Gene symbol KCNAB1
Gene ID (NCBI) 7881
RRIDAB_2131349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KCNAB1 antibody 14697-1-APDownload protocol
IHC protocol for KCNAB1 antibody 14697-1-APDownload protocol
IF protocol for KCNAB1 antibody 14697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols