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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNA6 Polyclonal antibody (31900-1-AP)

    WB analysis using 31900-1-AP (same clone as 31900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31900-1-AP (KCNA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNA6 Polyclonal antibody (31900-1-AP)

    IHC staining of mouse brain using 31900-1-AP (same clone as 31900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using KCNA6 Polyclonal antibody (31900-1-AP)

    IHC staining of rat brain using 31900-1-AP (same clone as 31900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using KCNA6 Polyclonal antibody (31900-1-AP)

    IF Staining of mouse cerebellum using 31900-1-AP (same clone as 31900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using KCNA6 antibody (31900-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

KCNA6 Polyclonal antibody, PBS Only

KCNA6 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31900-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31900-1-PBS targets KCNA6 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35654

Product name: Recombinant human KCNA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-262 aa of NM_002235.3

Sequence: ETLPQFRVDGRGGNNGGVSRVSPVSRGSQEEEEDEDDSYTFHHGITPGEMGTGGSSSLSTLGGSFFTDP

相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, shaker-related subfamily, member 6
Calculated molecular weight59 kDa，529aa
Observed molecular weight65 kDa
GenBank accession numberNM_002235.3
Gene Symbol KCNA6
Gene ID (NCBI) 3742
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP17658
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The Shaker-like Kv1 potassium channel family comprises 8 α subunits (Kv1.1-Kv1.8), which are key regulators of neuronal excitability in mammals (PMID: 7842011; 9581771; 22612818). Potassium voltage-gated channel subfamily A member 6 (KCNA6, also known as HBK2, Kv1.6, and PPP1R96) is a multi-pass membrane protein that is mainly expressed in the central and peripheral nervous system (PMID: 12042352; 8872310). The concentration of the Kv1.6 channel in the cell membrane can modulate the kinetic properties of Kv1.6-induced K+ current (PMID: 14575698).

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