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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCC4/SLC12A7 Polyclonal antibody (26590-1-AP)

    WB analysis using 26590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KCC4/SLC12A7 Polyclonal antibody (26590-1-AP)

    WB analysis using 26590-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 26590-1-AP (KCC4/SLC12A7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using KCC4/SLC12A7 Polyclonal antibody (26590-1-AP)

    IF Staining of A431 using 26590-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KCC4/SLC12A7 antibody (26590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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KCC4/SLC12A7 Polyclonal antibody

KCC4/SLC12A7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26590-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

K Cl cotransporter 4, KCC4, KCC4/SLC12A7, SLC12A7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBGC-823 cells, NCI-H1299 cells, Raji cells, SH-SY5Y cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26590-1-AP targets KCC4/SLC12A7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCC4/SLC12A7 fusion protein Ag24954 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium/chloride transporters), member 7
Observed molecular weight119 kDa
GenBank accession numberBC098390
Gene Symbol KCC4/SLC12A7
Gene ID (NCBI) 10723
RRIDAB_2918103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y666
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).

Protocols

Product Specific Protocols
WB protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
IF protocol for KCC4/SLC12A7 antibody 26590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols