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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCC2/SLC12A5 Polyclonal antibody

KCC2/SLC12A5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28724-1-AP

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

Validation Data Gallery

Applications:
  • WB analysis using 28724-1-AP

    WB analysis using 28724-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28724-1-AP (KCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 28724-1-AP

    IHC staining of rat brain using 28724-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC12A5 antibody included
  • IHC staining of rat brain using 28724-1-AP

    IHC staining of rat brain using 28724-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC12A5 antibody included
  • IHC staining of mouse brain using 28724-1-AP

    IHC staining of mouse brain using 28724-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC12A5 antibody included
  • IHC staining of mouse brain using 28724-1-AP

    IHC staining of mouse brain using 28724-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28724-1-AP (KCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC12A5 antibody included
  • IF Staining of mouse cerebellum using 28724-1-AP

    IF Staining of mouse cerebellum using 28724-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 28724-1-AP

    IF Staining of mouse cerebellum using 28724-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5 antibody (28724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28724-1-AP targets KCC2/SLC12A5 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCC2/SLC12A5 fusion protein Ag30203 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium-chloride transporter), member 5
Observed molecular weight130-150 kDa
GenBank accession numberBC132670
Gene symbol SLC12A5
Gene ID (NCBI) 57468
RRIDAB_2918195
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases.

Protocols

Product Specific Protocols
WB protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
IHC protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
IF protocol for KCC2/SLC12A5 antibody 28724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols