Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85382-1-RR targets KATNAL1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | KATNAL1 fusion protein Ag13960 相同性解析による交差性が予測される生物種 |
| Full Name | katanin p60 subunit A-like 1 |
| Calculated molecular weight | 490 aa, 55 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC000612 |
| Gene Symbol | KATNAL1 |
| Gene ID (NCBI) | 84056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BW62 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KATNAL1 belongs to a family of microtubule-severing proteins and contains an N-terminal microtubule interaction and trafficking (MIT) domain, an ATPase AAA-type core domain, and a C-terminal Vps4_C domain. KATNAL1 is involved in regulating microtubule dynamics, which is essential for spermiogenesis and male fertility. It severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays(PMID: 22654668). In neurons, KATNAL1 plays a role in microtubule rearrangement during neuronal migration and process elongation. Additionally, mutations in KATNAL1 have been associated with intellectual disability and microcephaly in humans(PMID: 30448058).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KATNAL1 antibody 85382-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |