Featured Product
KD/KO Validated

TIP60/KAT5 Polyclonal antibody

Name: TIP60/KAT5 Polyclonal antibody
Brand: Proteintech
SKU: 10827-1-AP

TIP60/KAT5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10827-1-AP

Synonyms

60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60

Validation Data Gallery

Applications:

AllWBIHC

WB analysis of HepG2 using 10827-1-AP
WB result of KAT5 antibody (10827-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAT5 transfected HepG2 cells.
WB analysis of PC-3 using 10827-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10827-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10827-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10827-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10827-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10827-1-AP (KAT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 10827-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10827-1-AP (KAT5 antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with KAT5 antibody included

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Cat No. 10827-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy KAT5 Ready-To-Use IHC Kit. KAT5 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

10827-1-AP targets TIP60/KAT5 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TIP60/KAT5 fusion protein Ag1261 相同性解析による交差性が予測される生物種
Full Name	K(lysine) acetyltransferase 5
Calculated molecular weight	60 kDa
Observed molecular weight	58 kDa, 70 kDa
GenBank accession number	BC000166
Gene symbol	KAT5
Gene ID (NCBI)	10524
RRID	AB_2128431
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa

Protocols

Product Specific Protocols
WB protocol for TIP60/KAT5 antibody 10827-1-AP	Download protocol
IHC protocol for TIP60/KAT5 antibody 10827-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature ANP32E is a histone chaperone that removes H2A.Z from chromatin. Authors - Arnaud Obri View Article
mouse	WB	Nat Immunol The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells. Authors - Jing Geng View Article
		Mol Cell PMID: 28366643 Authors - Sophie Barral View Article
human	WB	Nat Commun Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. Authors - Zhiyuan You KD Validated View Article
human	IF,IP	J Mol Cell Biol Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis Authors - Hameed Akbar View Article
human	WB	Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Authors - Heguo Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TIP60/KAT5 Polyclonal antibody

TIP60/KAT5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 10827-1-AP

WB analysis of PC-3 using 10827-1-AP

WB analysis of mouse liver using 10827-1-AP

WB analysis of HeLa using 10827-1-AP

WB analysis of rat liver using 10827-1-AP

WB analysis of Jurkat using 10827-1-AP

IHC staining of human prostate cancer using 10827-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells.

PMID: 28366643

Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.

Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

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