• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KARS Polyclonal antibody (14951-1-AP)

    WB analysis using 14951-1-AP (same clone as 14951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using KARS Polyclonal antibody (14951-1-AP)

    WB analysis of Jurkat using 14951-1-AP (same clone as 14951-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using KARS Polyclonal antibody (14951-1-AP)

    WB analysis of human colon using 14951-1-AP (same clone as 14951-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brown disease using KARS Polyclonal antibody (14951-1-AP)

    IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brown disease using KARS Polyclonal antibody (14951-1-AP)

    IHC staining of human brown disease using 14951-1-AP (same clone as 14951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14951-1-AP (KARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using KARS Polyclonal antibody (14951-1-AP)

    IF Staining of A431 using 14951-1-AP (same clone as 14951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KARS Polyclonal antibody, PBS Only

KARS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14951-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Lysyl-tRNA synthetase, Lysine--tRNA ligase, KIAA0070, KARS1, EC:2.7.7.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14951-1-PBS targets KARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6778

Product name: Recombinant human KARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 246-597 aa of BC004132

Sequence: IRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV

相同性解析による交差性が予測される生物種
Full Name lysyl-tRNA synthetase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC004132
Gene Symbol KARS
Gene ID (NCBI) 3735
RRIDAB_2128158
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15046
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KARS (lysyl-tRNA synthetase) is a gene that encodes an enzyme critical for protein synthesis, specifically responsible for attaching the amino acid lysine to its corresponding transfer RNA (tRNA) in both the cytoplasm and mitochondria. This enzyme plays a crucial role in the process of aminoacylation, which is essential for accurate protein translation from mRNA sequences. Mutations in the KARS gene have been associated with a variety of neurological and systemic disorders, reflecting the enzyme's importance in cellular function.

||
New chat

Able™

正在加载，请稍候...