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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JUNB Recombinant antibody (84922-6-RR)

    WB analysis using 84922-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84922-6-RR (JUNB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JUNB Recombinant antibody (84922-6-RR)

    IF Staining of HeLa using 84922-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (84922-6-RR, Clone: 242562G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using JUNB Recombinant antibody (84922-6-RR)

    FC experiment of HeLa using 84922-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug JUNB Recombinant antibody (84922-6-RR, Clone:242562G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84922-6-RR

    Affinity and Kinetic Characterization of 84922-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84922-6-RR against Human JUNB were performed. The affinity constant is 0.213 nM.

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JUNB Recombinant antibody

JUNB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84922-6-RR
Clone No.242562G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

AP-1, AP 1, 242562G2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84922-6-RR targets JUNB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen JUNB fusion protein Ag0752 相同性解析による交差性が予測される生物種
Full Name jun B proto-oncogene
Calculated molecular weight 36 kDa
Observed molecular weight 42 kDa, 43 kDa
GenBank accession numberBC004250
Gene Symbol JUNB
Gene ID (NCBI) 3726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Protocols

Product Specific Protocols
WB protocol for JUNB antibody 84922-6-RRDownload protocol
IF protocol for JUNB antibody 84922-6-RRDownload protocol
FC protocol for JUNB antibody 84922-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols