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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JTV1 Monoclonal antibody (66848-1-Ig)

    WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using JTV1 Monoclonal antibody (66848-1-Ig)

    WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using JTV1 Monoclonal antibody (66848-1-Ig)

    IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using JTV1 Monoclonal antibody (66848-1-Ig)

    IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using JTV1 Monoclonal antibody (66848-1-Ig)

    FC experiment of HepG2 using 66848-1-Ig (same clone as 66848-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug JTV1 Monoclonal antibody (66848-1-Ig, Clone:1D6B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

JTV1 Monoclonal antibody, PBS Only

JTV1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66848-1-PBS
Clone No.1D6B5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

1D6B5, AIMP2, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, HLD17, P38

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66848-1-PBS targets JTV1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0675

Product name: Recombinant human JTV1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC002853

Sequence: MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFS

相同性解析による交差性が予測される生物種
Full Name JTV1 gene
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC002853
Gene Symbol JTV1
Gene ID (NCBI) 7965
RRIDAB_2882188
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13155
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

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