Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 10424-1-AP
WB result of JTV1 antibody (10424-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-JTV1 transfected HeLa cells.
WB analysis using 10424-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10424-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10424-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10424-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 10424-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 10424-1-AP (JTV1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10424-1-AP
IP result of anti-JTV1 (IP:10424-1-AP, 3ug; Detection:10424-1-AP 1:1000) with HeLa cells lysate 800ug.
IHC staining of mouse cerebellum using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10424-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10424-1-AP (JTV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10424-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using JTV1 antibody (10424-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 10424-1-AP
Immunofluorescent analysis of HepG2 cells, using JTV1 antibody 10424-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

JTV1 Polyclonal antibody

JTV1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10424-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Protein JTV-1, PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (29)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, rat liver tissue, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse cerebellum tissue, human kidney tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 26 publications below
IHC	See 3 publications below
IF	See 8 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

10424-1-AP targets JTV1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0675 Product name: Recombinant human JTV1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC002853 Sequence: MPMYQVKPYHGGGAPLRVELPTCMYRLPNVHGRSYGPAPGAGHVQEESNLSLQALESRQDDILKRLYELKAAVDGLSKMIQTPDADLDVTNIIQADEPTTLTTNALDLNSVLGKDYGALKDIVINANPASPPLSLLVLHRLLCEHFRVLSTVHTHSSVKSVPENLLKCFGEQNKKQPRQDYQLGFTLIWKNVPKTQMKFS 相同性解析による交差性が予測される生物種
Full Name	JTV1 gene
Calculated molecular weight	36 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC002853
Gene Symbol	JTV1
Gene ID (NCBI)	7965
RRID	AB_513880
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13155
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. This is a rabbit polyclonal antibody raised against full-length of JTV1. There're two isoforms of JTV1 with MW about 27 kDa and 35 kDa,

Protocols

Product Specific Protocols
IF protocol for JTV1 antibody 10424-1-AP	Download protocol
IHC protocol for JTV1 antibody 10424-1-AP	Download protocol
IP protocol for JTV1 antibody 10424-1-AP	Download protocol
WB protocol for JTV1 antibody 10424-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Global landscape of HIV-human protein complexes. Authors - Stefanie Jäger View Article
human	WB,IP	Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation Authors - Raja H Ali View Article
mouse	WB,IF	Nat Neurosci Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss. Authors - Lee Yunjong Y View Article
human	WB	J Exp Clin Cancer Res RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization Authors - Shuang Tao View Article
human	WB,IF	Nat Commun Molecular determinants of selective clearance of protein inclusions by autophagy. Authors - Wong Esther E View Article
mouse	WB	Proc Natl Acad Sci U S A Role for the obesity-related FTO gene in the cellular sensing of amino acids. Authors - Gulati Pawan P View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10424-1-AP

WB analysis using 10424-1-AP

WB analysis of HeLa using 10424-1-AP

WB analysis of NIH/3T3 using 10424-1-AP

WB analysis of K-562 using 10424-1-AP

WB analysis of rat liver using 10424-1-AP

IP experiment of HeLa using 10424-1-AP

IHC staining of mouse cerebellum using 10424-1-AP

IHC staining of mouse cerebellum using 10424-1-AP

IHC staining of human kidney using 10424-1-AP

IHC staining of human kidney using 10424-1-AP

IHC staining of mouse testis using 10424-1-AP

IHC staining of mouse testis using 10424-1-AP

IF Staining of HeLa using 10424-1-AP

IF Staining of HepG2 using 10424-1-AP

JTV1 Polyclonal antibody

JTV1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Global landscape of HIV-human protein complexes.

A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation

Parthanatos mediates AIMP2-activated age-dependent dopaminergic neuronal loss.

RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization

Molecular determinants of selective clearance of protein inclusions by autophagy.

Role for the obesity-related FTO gene in the cellular sensing of amino acids.